Single-molecule optical genome mapping of a human HapMap and a colorectal cancer cell line

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Table 3 Summary of MapCard statistics of HCT116

MapCard ID	F^a	Input maps^b (theoretical genome coverage)	Average Argus quality score	Average DNA molecule size (kb)	Average # of fragments	Average fragment size (kb)	OPTIMA alignment rate	Yield (genome coverage)^c	Average digestion rate^c	Average false/ extra cut rate^c	Ratio small missing fragments (≤2 kb)^c
17182LA	(r)	10911 (0.9×)	0.33	257	16	15.7	0.040	0.04×	0.661	1.288	0.170
	(s)	3744 (0.4×)	0.33	351	20	17.7	0.040	0.02×	0.628	1.226	0.190
17184LA-2	(r)	55719 (5.7×)	0.43	305	19	16.3	0.180	1.1×	0.678	0.760	0.197
	(s)	28658 (3.7×)	0.45	390	23	17.2	0.250	0.9×	0.669	0.737	0.199
17185LA	(r)	56879 (5.4×)	0.55	285	19	14.7	0.240	1.5×	0.705	0.756	0.219
	(s)	28003 (3.4×)	0.59	365	24	15.1	0.352	1.2×	0.696	0.739	0.217
17186LA-3	(r)	52984 (5.8×)	0.54	328	20	16.0	0.327	2.0×	0.696	0.677	0.167
	(s)	31588 (4.3×)	0.56	404	25	16.4	0.423	1.7×	0.688	0.671	0.163
17187LA	(r)	88730 (7.8×)	0.45	264	18	14.8	0.115	1.0×	0.692	0.940	0.195
	(s)	36018 (4.2×)	0.46	349	22	15.8	0.171	0.7×	0.678	0.919	0.188
14593LB	(r)	30994 (2.7×)	0.39	261	14	18.9	0.059	0.2×	0.626	0.847	0.161
	(s)	10944 (1.2×)	0.39	337	17	20.2	0.086	0.1×	0.597	0.869	0.151
Total	(r)	296217 (28.3×)	0.47	287	18	15.7	0.181	5.7×	0.691	0.774	0.191
	(s)	138955 (17.2×)	0.50	372	23	16.5	0.271	4.6×	0.682	0.749	0.188

^ar: inclusion of DNA molecules with ≥10 fragments and ≥150 kb in length; s: inclusion of DNA molecules with ≥12 fragments and ≥250 kb in length
^bfragmented DNA molecules
^cof OPTIMA aligned data

ISSN: 2047-217X